Journal: Cardiovascular Research

Article Title: Regulation of Ptbp1-controlled alternative splicing of pyruvate kinase muscle by liver kinase B1 governs vascular smooth muscle cell plasticity in vivo

doi: 10.1093/cvr/cvae187

Figure Lengend Snippet: Histological analysis of the aortas from WT and Lkb1 SMiKO mice. ( A ) H&E, elastin, and Masson trichrome stains of the thoracic aortas from WT and Lkb1 SMiKO mice at 1.0–1.5 months post-TAM induction. Scale bars: 200 µm. ( B ) H&E staining and immunofluorescence staining (α-actin) of the thoracic aortas from WT and Lkb1 SMiKO mice at 1.0–1.5 months post-TAM induction. Scale bars: 20 and 50 µm, respectively. ( C ) Quantification of the medial wall thickness of the thoracic aortas from WT and Lkb1 SMiKO mice at 1.5 months post-TAM induction. Data represent mean ± SEM ( n = 6 for WT mice and n = 8 for Lkb1 SMiKO mice). ( D ) Quantification of the internal and external elastic laminae (IEL and EEL, respectively) perimeters of the thoracic aortas from WT and Lkb1 SMiKO mice at 1.5 and 4.5 months post-TAM induction. Data represent mean ± SEM ( n = 6 for WT mice and n = 8 for Lkb1 SMiKO mice). ( E ) H&E, elastin, and Masson stains of the thoracic aortas from WT and Lkb1 SMiKO mice at 4.5 months post-TAM induction. Scale bars: 200 µm. Boxes indicate fields shown at higher magnification in images to the immediate right. ( F ) Alcian Blue, Safranin O, and Von Kossa staining of the thoracic aortas from WT and Lkb1 SMiKO mice at 4.5 months post-TAM induction. Scale bars: 200 µm. Boxes indicate fields shown at higher magnification in images to the immediate right. ( G and H ) Immunofluorescence staining for α-actin ( G ), SM22α ( G ), and Col2a1 ( H ) of the thoracic aortas from WT and Lkb1 SMiKO mice at 4.5 months post-TAM induction ( n = 6 mice per genotype). Where indicated, nuclei were counterstained with DAPI. Dashed lines denote the IEL and EEL of the aortas. Scale bars: 200 µm. Boxes indicate fields shown at higher magnification in images to the immediate right. P -values were determined using Student’s t -test ( C and D ). For all panels, * P < 0.05; ** P < 0.01.

Article Snippet: Briefly, OCT-embedded sections were washed with phosphate-buffered saline (PBS), fixed with acetone at 4°C for 15 min, and permeabilized with 0.2% Triton X-100 for 10 min. After blocking with goat serum (protein block) for 30 min, the sections were incubated with primary antibodies against α-actin (Abcam, Cat #ab7817, 1:200), SM22α (Abcam, Cat #ab14106, 1:200), Col2A1 (Boster, Cat #PA2141-1, 1:200), and Sox9 (Millipore, Cat #ABE2868, 1:500) overnight.

Techniques: Staining, Immunofluorescence

Journal: Stem Cell Research & Therapy

Article Title: Characterization of bursa subacromialis-derived mesenchymal stem cells

doi: 10.1186/s13287-015-0104-3

Figure Lengend Snippet: Primer sequences and PCR conditions

Article Snippet: Immunohistochemical detection of collagen type II (COL II) was performed as described in detail previously [ ] using a primary monoclonal COL II antibody (Acris Antibodies GmbH, Herford, Germany), while negative controls were treated with mouse serum instead.

Techniques: Sequencing, Marker

Journal: Stem Cell Research & Therapy

Article Title: Characterization of bursa subacromialis-derived mesenchymal stem cells

doi: 10.1186/s13287-015-0104-3

Figure Lengend Snippet: Chondrogenic differentiation of BS cells and BMSCs. Cells cultivated in chondrogenic medium showed strong staining for proteoglycans determined by positive alcian blue staining (Alc Blue) and were also positive for collagen type II (COL II) in BS cells as well as in BMSC compared to cells cultivated in control medium ( a ). Left scale bar = 200 μm; right scale bar = 100 μm. b Expression of chondrogenic marker genes was evaluated using RT-PCR. Cultivation in the presence of chondrogenic medium (Ch) resulted in an increased expression of aggrecan (AGN), decorin (DEC), SRY (sex determining region Y)-box 9 (SOX9), indian hedgehog (IHH), and collagen type II (COL II) as compared to untreated cells (Co) for both cell types. Expression levels of the housekeeping gene elongation factor 1α (EF1α) are shown in the last row. Representative images from three different donors are shown. BMSCs bone-marrow derived mesenchymal stem cells BS bursa subacromialiss

Article Snippet: Immunohistochemical detection of collagen type II (COL II) was performed as described in detail previously [ ] using a primary monoclonal COL II antibody (Acris Antibodies GmbH, Herford, Germany), while negative controls were treated with mouse serum instead.

Techniques: Staining, Expressing, Marker, Reverse Transcription Polymerase Chain Reaction, Derivative Assay

Journal: Bioengineering

Article Title: Comparison of Bioengineered Scaffolds for the Induction of Osteochondrogenic Differentiation of Human Adipose-Derived Stem Cells

doi: 10.3390/bioengineering11090920

Figure Lengend Snippet: hASC survival and differentiation in GelMA hydrogels. ( A ) CCK8 assay of hASCs encapsulated in the GelMA hydrogel at different time points (1 week intervals). Results are reported as mean ± SEM of n = 3 samples/group. ( B ) Representative confocal imaging of Dead/live fluorescence staining of hASCs in the GelMA hydrogel after 3 weeks of culture. Red cells are dead while green cells are alive. ( C ) Alcian blue staining of GelMA hydrogel after 24 h and 3 weeks of culture. ( D ) Alizarin red staining of GelMA hydrogel after 24 h and 3 weeks of culture. ( E , F ) Confocal imaging of immunofluorescence for COL2A1 (green) and OCN (red) in hASCs encapsulated in GelMA hydrogel after 3 weeks of culture.

Article Snippet: Briefly, samples were incubated with rabbit polyclonal anti-collagen type II alpha 1 (COL2A1) antibody (Merck) and rabbit polyclonal anti-osteocalcin (OCN) antibody (Thermo Fisher Scientific) for 1 h at room temperature.

Techniques: CCK-8 Assay, Imaging, Fluorescence, Staining, Immunofluorescence

Journal: Bioengineering

Article Title: Comparison of Bioengineered Scaffolds for the Induction of Osteochondrogenic Differentiation of Human Adipose-Derived Stem Cells

doi: 10.3390/bioengineering11090920

Figure Lengend Snippet: hASC survival and differentiation in PEGDA scaffold. ( A ) CCK8 assay of hASCs seeded in the PEGDA scaffold at different time points (1 week intervals). Results are reported as mean ± SEM of n = 3 samples/group. T test: * p < 0.05. ( B ) Representative confocal imaging of Dead/live fluorescence staining of hASCs seeded in the PEGDA scaffold after 3 weeks of culture. ( C ) Confocal imaging of immunofluorescence for COL2A1 (green) and OCN (red) in hASCs seeded in the PEGDA scaffold after 3 weeks of culture.

Article Snippet: Briefly, samples were incubated with rabbit polyclonal anti-collagen type II alpha 1 (COL2A1) antibody (Merck) and rabbit polyclonal anti-osteocalcin (OCN) antibody (Thermo Fisher Scientific) for 1 h at room temperature.

Techniques: CCK-8 Assay, Imaging, Fluorescence, Staining, Immunofluorescence

Journal: Bioengineering

Article Title: Comparison of Bioengineered Scaffolds for the Induction of Osteochondrogenic Differentiation of Human Adipose-Derived Stem Cells

doi: 10.3390/bioengineering11090920

Figure Lengend Snippet: hASC survival and differentiation in celery-based scaffold. ( A ) SEM imaging of a single hASC inside a niche of the celery-based scaffold. ( B ) CCK8 assay of hASCs seeded in the celery-based scaffold at different time points (1 week intervals). Results are reported as mean ± SEM of n = 3 samples/group. T test: * p < 0.05. ( C ) Representative confocal imaging of Dead/live fluorescence staining of hASCs seeded in the scaffold after 3 weeks of culture. ( D ) Confocal 3D stack and ( E ) the projection of hASC distribution inside the scaffold. ( F ) Confocal imaging of immunofluorescence for COL2A1 (green) and OCN (red) in hASCs seeded in the PEGDA scaffold after 3 weeks of culture.

Article Snippet: Briefly, samples were incubated with rabbit polyclonal anti-collagen type II alpha 1 (COL2A1) antibody (Merck) and rabbit polyclonal anti-osteocalcin (OCN) antibody (Thermo Fisher Scientific) for 1 h at room temperature.

Techniques: Imaging, CCK-8 Assay, Fluorescence, Staining, Immunofluorescence

Journal: Veterinary Medicine International

Article Title: Articular Cartilage Gene Expression after Coxofemoral Joint Luxation in the Dog

doi: 10.1155/2013/936317

Figure Lengend Snippet: Sequences of sense and antisense primers used for amplification in real-time PCR.

Article Snippet: Quantification of ten transcripts—aggrecan ( AGG ); type II collagen, alpha-1 chain ( COL2A1 ); matrix metalloproteinase-3 ( MMP-3 ); hyaluronan synthase-1 ( HAS-1 ); hyaluronan synthase-2 ( HAS-2 ); tissue inhibitor of metalloproteinase-1 ( TIMP-1 ); Bcl-2-associated X protein ( BAX ); B-cell lymphoma-2 ( BCL-2 ); cysteine aspartate-specific protease-3 ( CAS-3 ); and cysteine aspartate-specific protease-9 ( CAS-9 ) (Shanghai BlueGene Biotech, Shanghai, China)—was performed for all samples.

Techniques: Amplification, Sequencing

Journal: Veterinary Medicine International

Article Title: Articular Cartilage Gene Expression after Coxofemoral Joint Luxation in the Dog

doi: 10.1155/2013/936317

Figure Lengend Snippet: The relationship between relative expression of nonapoptotic genes and days of luxation ( AGG , aggrecan; COL2A , type II collagen (alpha-1 chain); HAS-1 , hyaluronan synthase-1; HAS-2 , hyaluronan synthase-2; TIMP , tissue inhibitor of metalloproteinase; MMP-3 , matrix metalloproteinase-3).

Article Snippet: Quantification of ten transcripts—aggrecan ( AGG ); type II collagen, alpha-1 chain ( COL2A1 ); matrix metalloproteinase-3 ( MMP-3 ); hyaluronan synthase-1 ( HAS-1 ); hyaluronan synthase-2 ( HAS-2 ); tissue inhibitor of metalloproteinase-1 ( TIMP-1 ); Bcl-2-associated X protein ( BAX ); B-cell lymphoma-2 ( BCL-2 ); cysteine aspartate-specific protease-3 ( CAS-3 ); and cysteine aspartate-specific protease-9 ( CAS-9 ) (Shanghai BlueGene Biotech, Shanghai, China)—was performed for all samples.

Techniques: Expressing

Journal: Veterinary Medicine International

Article Title: Articular Cartilage Gene Expression after Coxofemoral Joint Luxation in the Dog

doi: 10.1155/2013/936317

Figure Lengend Snippet: Linear regression equation R , R 2 , and significance level (Sig.) of candidate genes.

Article Snippet: Quantification of ten transcripts—aggrecan ( AGG ); type II collagen, alpha-1 chain ( COL2A1 ); matrix metalloproteinase-3 ( MMP-3 ); hyaluronan synthase-1 ( HAS-1 ); hyaluronan synthase-2 ( HAS-2 ); tissue inhibitor of metalloproteinase-1 ( TIMP-1 ); Bcl-2-associated X protein ( BAX ); B-cell lymphoma-2 ( BCL-2 ); cysteine aspartate-specific protease-3 ( CAS-3 ); and cysteine aspartate-specific protease-9 ( CAS-9 ) (Shanghai BlueGene Biotech, Shanghai, China)—was performed for all samples.

Techniques: